Tsang A Y, Lee W M, Wong P Y
Int J Androl. 1981 Dec;4(6):703-12. doi: 10.1111/j.1365-2605.1981.tb00754.x.
The possibility that alpha-chlorohydrin, 6-chloro-6-deoxyglucose (6CDG) and cyproterone acetate (CPA) might affect epididymal protein secretion or acquisition of sperm surface proteins as the cause of their antifertility action in male rats was investigated. Daily administration of 9 mg/kg alpha-chlorohydrin for 7--14 days and 24 mg/kg 6CDG for 14--21 days induced sterility in male rats and imparied the capacity of the cauda epididymal spermatozoa to initiate motility. Treatment with CPA (30 mg/kg/day) for 21--28 days, however, was found to have no effect on fertility and initiation of sperm motility, although the epididymis of the treated animals underwent a loss in weight. The antifertility effects of alpha-chlorohydrin or 6CDG did not seem to be attributed to an interference with epididymal protein secretion. The cauda epididymal fluids of the alpha-chlorohydrin, 6CDG and CPA treated animals have similar protein patterns compared to those of the control animals. However, when the surface proteins of the spermatozoa were labelled with radioactive iodine, the sperm surface proteins alpha-chlorohydrin and 6CDG treated animals were found to differ from those of the control animals. Two peaks (MW 32 000 and 70 000) and one peak (70 000) were significantly reduced in the alpha-chlorohydrin treated and 6CDG treated animals, respectively. Additional bands appeared on the surface of the treated (infertile) animals. In contrast, CPA treatment did not affect the surface protein pattern of the epididymal spermatozoa. It was concluded that the antifertility affects of alpha-chlorohydrin and 6CDG are not due to an interference with epididymal secretion of specific proteins but to an intervention of the subsequent acquisition of these proteins by epididymal spermatozoa. This results in a decrease in the capacity of the epididymal sperm to initiate motility and hence a loss of fertilizing capacity.
研究了α-氯醇、6-氯-6-脱氧葡萄糖（6CDG）和醋酸环丙孕酮（CPA）可能影响附睾蛋白质分泌或精子表面蛋白质获得，从而导致其对雄性大鼠抗生育作用的可能性。每日给予9mg/kgα-氯醇，持续7 - 14天，以及给予24mg/kg 6CDG，持续14 - 21天，可使雄性大鼠不育，并损害附睾尾部精子启动运动的能力。然而，发现用CPA（30mg/kg/天）治疗21 - 28天对生育力和精子运动启动没有影响，尽管治疗动物的附睾重量减轻。α-氯醇或6CDG的抗生育作用似乎并非归因于对附睾蛋白质分泌的干扰。与对照动物相比，用α-氯醇、6CDG和CPA处理的动物的附睾尾部液体具有相似的蛋白质模式。然而，当用放射性碘标记精子的表面蛋白质时，发现用α-氯醇和6CDG处理的动物的精子表面蛋白质与对照动物不同。在α-氯醇处理的动物和6CDG处理的动物中，分别有两个峰（分子量32000和70000）和一个峰（70000）显著减少。在处理过的（不育）动物的表面出现了额外的条带。相比之下，CPA处理不影响附睾精子的表面蛋白质模式。得出的结论是，α-氯醇和6CDG的抗生育作用不是由于干扰附睾特定蛋白质的分泌，而是由于附睾精子随后对这些蛋白质的获得受到干预。这导致附睾精子启动运动的能力下降，从而丧失受精能力。
