使用茚三酮对高效液相色谱中的胍基化合物进行柱后衍生化。
Post-column derivatization of guanidino compounds in high-performance liquid chromatography using ninhydrin.
作者信息
Hiraga Y, Konoshita T
出版信息
J Chromatogr. 1981 Nov 13;226(1):43-51. doi: 10.1016/s0378-4347(00)84204-8.
A new method for the high-performance liquid chromatography of guanidino compounds using ninhydrin as the fluorescence reagent is described. Use of organic solvent is not required since ninhydrin is highly soluble in aqueous media, and the problem of precipitation formation occurring in the phenanthrenequinone method was avoided. Creatine was also assayed in the present procedure. Separation of ten guanidino compounds was completed within 30 min using a small-size (38 X 4.2 mm I.D.) strong cation-exchange column.
描述了一种使用茚三酮作为荧光试剂的胍基化合物高效液相色谱新方法。由于茚三酮在水性介质中高度可溶，因此无需使用有机溶剂，并且避免了菲醌法中出现的沉淀形成问题。本方法还对肌酸进行了测定。使用小尺寸（内径38×4.2 mm）强阳离子交换柱，在30分钟内完成了十种胍基化合物的分离。
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