Sato K, Ishida K, Kuno T, Mizuno A, Shimizu S
J Nutr Sci Vitaminol (Tokyo). 1981;27(5):439-47. doi: 10.3177/jnsv.27.439.
The regulation of vitamin B12 and bacteriochlorophyll formation was studied in a facultative methylotroph, Protaminobacter ruber, classified as a non-photosynthetic bacterium. Vitamin B12 was formed at an almost constant level under various cultivation conditions, while bacteriochlorophyll synthesis was drastically influenced by the growth conditions. Addition of B12 and hemin to the medium and the change of culture conditions from light to darkness in the early growth phases stimulated the pigment synthesis, but no pigment was formed under the continuous illumination and the addition of chloramphenicol inhibited the pigment formation. delta-ALA synthase and delta-ALA dehydratase activities were demonstrated in P. ruber. delta-ALA synthase formation was induced when cultures were transferred from light to dark conditions, which promoted the bacteriochlorophyll formation. Bacteriochlorophyll-protein complex had an absorption maximum at 870 nm and a very small peak at 800 nm.
在一种被归类为非光合细菌的兼性甲基营养菌——鲁氏原杆菌（Protaminobacter ruber）中，研究了维生素B12和细菌叶绿素形成的调控。在各种培养条件下，维生素B12以几乎恒定的水平形成，而细菌叶绿素的合成则受到生长条件的显著影响。向培养基中添加B12和血红素，以及在生长早期将培养条件从光照改为黑暗，都刺激了色素的合成，但在持续光照下不形成色素，添加氯霉素则抑制色素形成。在鲁氏原杆菌中证实了δ-氨基乙酰丙酸（δ-ALA）合酶和δ-ALA脱水酶的活性。当培养物从光照条件转移到黑暗条件时，诱导了δ-ALA合酶的形成，这促进了细菌叶绿素的形成。细菌叶绿素-蛋白质复合物在870nm处有最大吸收峰，在800nm处有一个非常小的峰。
