Oikarinen J, Ryhänen L
Biochem J. 1981 Sep 15;198(3):519-24. doi: 10.1042/bj1980519.
The calvarial mRNA species of chick embryos were translated in the rabbit reticulocyte-lysate cell-free translation system. The amount of procollagen type-I mRNA species was determined by digestion with bacterial collagenase and by fluorography of the cell-free translation products. Administration of cortisol resulted in a specific decrease in the cellular concentration of translatable procollagen type-I mRNA species in the calvaria of developing chick embryos. There was a lag period of up to 12 h before the response, which was dose-dependent. The data suggest that the decrease in amounts of procollagen mRNA species is the main reason for the lower amount of tissue collagen after topical or systemic administration of glucocorticoids, although other factors may contribute to the response.
鸡胚颅骨的mRNA种类在兔网织红细胞裂解物无细胞翻译系统中进行翻译。通过用细菌胶原酶消化以及对无细胞翻译产物进行荧光显影来测定I型前胶原mRNA种类的量。给予皮质醇导致发育中的鸡胚颅骨中可翻译的I型前胶原mRNA种类的细胞浓度特异性降低。在反应之前有长达12小时的延迟期，该反应呈剂量依赖性。数据表明，I型前胶原mRNA种类数量的减少是局部或全身给予糖皮质激素后组织胶原蛋白量降低的主要原因，尽管其他因素可能也对该反应有影响。
