无机磷酸盐在线粒体能量转导的化学渗透机制中的参与。

Participation of inorganic phosphate in the chemiosmotic mechanism of mitochondrial energy transduction.

作者信息

Fonyó A, Lukács G, Ligeti E

出版信息

Acta Biol Med Ger. 1981;40(7-8):907-14.

Abstract

In respiring liver mitochondria in which Pi transport was inhibited and the intramitochondrial Pi value manipulated to vary about 3-fold, K+-ion uptake, H+-ion extrusion and stimulation of respiration were linearly related to the intramitochondrial Pi content. It is suggested that the HPO 2(4)-anion generated during respiration is the nondiffusible anion required for the Donnan distribution and membrane potential in mitochondria. The intramitochondrial H X HPO4-ionic species is the proton donor required for coupled respiration. The electroneural Pi-H+ cotransport closes the proton cycling in respiration.

摘要

在呼吸作用的肝线粒体中，无机磷酸（Pi）转运受到抑制，线粒体内Pi值被调控在约3倍范围内变化，钾离子摄取、氢离子外排以及呼吸刺激与线粒体内Pi含量呈线性相关。有观点认为，呼吸过程中产生的磷酸氢根离子（HPO₂(₄)⁻）是线粒体中唐南分布和膜电位所需的非扩散性阴离子。线粒体内的磷酸二氢根离子（H₂PO₄⁻）是偶联呼吸所需的质子供体。电中性的Pi-H⁺协同转运完成了呼吸作用中的质子循环。