Albanese-Carmignani M P, Zaccone G
Acta Histochem. 1977;59(2):273-84. doi: 10.1016/s0065-1281(77)80050-0.
Recent cytochemical techniques for the detection of proteins, neutral and acid mucopolysaccharides, lipids and phospholipids in the yolk platelets of the growing oocytes of Rana esculenta and Triturus vulgaris are employed. Cytochemical analysis showed that the yolk in the form of granules or platelets, in the constituting itself the most prominent cytoplasmic inclusion of amphibian eggs, contains a protein moiety consisting of individual typical amino acids lacking tryptophan in Triturus, a carbohydrate fraction consisting of neutral mucopolysaccharides distributed in the superficial layer of each platelet, and phospholipids, phosphoproteins and lipoproteins which are present only in the main body of the same platelets. The different amino acid composition indicated a protein heterogeneity which could be connected with the tissue incompatibility of the transplantations when these last were effected between the orders Urodela and Anura. The different protein composition of the yolk also can be reflected at the species-specific level. The cytochemical data are further reviewed with the mode of utilization of the yolk platelets.
采用了近期的细胞化学技术来检测食用蛙和普通蝾螈生长中卵母细胞的卵黄小板中的蛋白质、中性和酸性粘多糖、脂质及磷脂。细胞化学分析表明，呈颗粒或小板形式的卵黄，作为两栖类卵中最显著的细胞质内含物，包含一个蛋白质部分，该部分由普通蝾螈中缺乏色氨酸的个别典型氨基酸组成；一个碳水化合物部分，由分布在每个小板表层的中性粘多糖组成；以及仅存在于同一小板主体中的磷脂、磷蛋白和脂蛋白。不同的氨基酸组成表明蛋白质具有异质性，当在有尾目和无尾目之间进行移植时，这种异质性可能与移植的组织不相容性有关。卵黄不同的蛋白质组成也可在物种特异性水平上得到体现。结合卵黄小板的利用方式对细胞化学数据进行了进一步综述。
