边缘无形体的冷冻干燥
Freeze-drying of Anaplasma marginale.
作者信息
Potgieter F T, Bester J B
出版信息
Onderstepoort J Vet Res. 1981 Sep;48(3):179-80.
PMID:7335334
Abstract
Heparinized whole blood, heavily parasitized with Anaplasma marginale, was collected from 3 splenectomized oxen. Buffered lactose peptone (BLP) was added to equal volumes as a stabilizer and the mixture lyophilized in 2 ml aliquots after rapid freezing. The dried material was reconstituted with 2 ml sterile water and inoculated without delay. The product remained infective for at least 6 months when stored in an ordinary household deep-freeze unit.
摘要
从3头脾切除的牛身上采集了感染了边缘无形体的肝素化全血。加入等量的缓冲乳糖蛋白胨（BLP）作为稳定剂，快速冷冻后将混合物以2毫升的等分试样进行冻干。将干燥后的材料用2毫升无菌水复溶并立即接种。当储存在普通家用冰柜中时，该产品至少6个月仍具有感染性。
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