The influence of bradykinin in vitro on uptake and release of dopamine by rat striatal synaptosomes.

Bradykinin differently affected the uptake of 3H-dopamine (assayed in synaptosomes separated by two methods: centrifugation and filtration) under various conditions of amine and investigated peptide concentration. 3H-dopamine uptake (assayed with the method of centrifugation) was depressed by cocaine, and bradykinin potentiated this effect. Similarly ouabain inhibited the dopamine uptake and this effect was potentiated by bradykinin. Bradykinin did not influence or potentiated the release of tritium from synaptosomes previously incubated with 3H-dopamine. The results indicate that bradykinin modifies the uptake and release of dopamine in rat striatal synaptosomes.