人类红细胞生成分化：关于胎儿期、新生儿期及成年期红细胞祖细胞和血红蛋白合成的体外研究

Erythropoietic differentiation in humans: in vitro studies on erythroid progenitors and Hb synthesis in fetal, newborn and adult life.

作者信息

Peschle C, Migliaccio G, Migliaccio A R, Lettieri F, Russo G, Gianni A M, Ottolenghi S, Comi P, Giglioni B

出版信息

Exp Hematol. 1980;8 Suppl 8:153-9.

Abstract

Human erythroid progenitors from fetal liver, cord or adult blood and adult marrow were cultured in methylcellulose, according to standard techniques. Their clonogenetic features (colony morphology and number, time/growth curve, erythropoietin (Ep) and burst-enhancing factor (BEF) sensitivity, in vitro 3H-thymidine suicide index, etc) were comparatively investigated. Three classes of fetal liver erythroid progenitors (primitive or intermediate BFU-E, CFU-E) have been thereby identified and characterized. Furthermore, globin chains (alpha, beta, G gamma, A gamma) synthesis has been evaluated in single erythroid colonies, either well-or poorly-hemoglobinized, by means of a novel technique including analytical iso-electric focusing (IEF), sometimes preceded by preparative IEF separation of HbF and HbA. On the basis of these results, a model for the regulation of Hb synthesis is proposed here.

摘要

按照标准技术，将来自胎儿肝脏、脐带血或成人血液及成人骨髓的人类红系祖细胞培养于甲基纤维素中。对它们的克隆形成特征（集落形态和数量、时间/生长曲线、促红细胞生成素（Ep）和爆式增强因子（BEF）敏感性、体外3H-胸腺嘧啶核苷自杀指数等）进行了比较研究。由此鉴定并表征了三类胎儿肝脏红系祖细胞（原始或中间型爆式形成单位-红细胞（BFU-E）、红细胞生成单位（CFU-E））。此外，通过一种新技术，包括分析性等电聚焦（IEF），有时在对HbF和HbA进行制备性IEF分离之后，对单个红系集落中珠蛋白链（α、β、Gγ、Aγ）的合成进行了评估，这些集落的血红蛋白化程度有高有低。基于这些结果，本文提出了一个血红蛋白合成调控模型。