Effect of antibiotics, non-ionic detergents, and vitamins on the osmotic barriers of Micrococcus glutamicus.

The production of amino acids by the mutant strain, homoserine methionine deficient, of Micrococcus glutamicus, was studied through the elucidation of the role of chemical agent affecting the osmotic barriers. Penicillin was found to affect the cell wall integrity and to increase greatly the total amino acid content, especially in presence of high biotin content. Non-ionic detergents were found to affect the integrity of cytoplasmic membrane. The effect was not similar with the different types of detergents and not proportional to the increase of its concentration.