[兔分泌型免疫球蛋白A在pH稳定梯度中的等电聚焦]

[Isoelectric focusing in pH stable gradient of secretory immunoglobulin A of rabbits].

作者信息

Ovod V V, Kulik G V

出版信息

Ukr Biokhim Zh (1978). 1980 Jan-Feb;52(1):52-7.

Abstract

Rabbit secretory immunoglobulin A was focused in the ampholine system (pH 3-10, 4-6). The focusing of a 25-30 mg sample in the medium with a low ionic strength is accompanied by an intensive precipitation of immunoglobulin A at isopoints. The immunoglobulin A-ampholine complex is formed, protein molecules acquire a negative charge and migrate to the anode. The focusing of 10-15 mg protein in the medium with 2.10(-3) NaCl stabilizes the protein under study at isopoints. The immunoglobulin distributes in a zone of the isoelectric spectrum of pH 15-4.30 units with total pI 4.75.

摘要

兔分泌型免疫球蛋白A在两性电解质系统（pH 3 - 10，4 - 6）中进行聚焦。在低离子强度介质中对25 - 30毫克样品进行聚焦时，免疫球蛋白A在等电点处会强烈沉淀。形成免疫球蛋白A - 两性电解质复合物，蛋白质分子带负电荷并向阳极迁移。在含有2×10⁻³氯化钠的介质中对10 - 15毫克蛋白质进行聚焦，可使所研究的蛋白质在等电点处稳定。该免疫球蛋白分布在pH 15 - 4.30单位的等电谱区域，总pI为4.75。