Kolodzĭskaia M V, Verbilenko S V, Konoplich L A
Ukr Biokhim Zh (1978). 1980 Jan-Feb;52(1):92-6.
It is shown that iodacetate and iodacetamide produce an insignificant inhibition of the Asp. oryzae aminopeptidase activity, para-chloromercuribenzoate is a stronger inhibitor. Dithiotreitol, beta-mercaptoethanol, reduced glutathione also cause a considerable loss in the enzyme activity. The inhibitory effect is intensified with a combined action of para-chloromercuribenzoate and EDTA. The studies of para-chloromercuribenzoate, iodacetate and iodacetamide effect on the activation of aminopeptidase apoenzyme by cobalt ions showed that the enzyme activity recovery is insignificant. It may be supposed that the enzyme thiol groups are bound with the metal ions necessary for the catalytic activity.
结果表明，碘乙酸盐和碘乙酰胺对米曲霉氨肽酶活性的抑制作用不明显，对氯汞苯甲酸是一种更强的抑制剂。二硫苏糖醇、β-巯基乙醇、还原型谷胱甘肽也会导致该酶活性显著丧失。对氯汞苯甲酸和EDTA联合作用会增强抑制效果。对氯汞苯甲酸、碘乙酸盐和碘乙酰胺对钴离子激活氨肽酶脱辅酶作用的研究表明，酶活性的恢复不明显。可以推测，该酶的巯基与催化活性所需的金属离子结合。
