Klein R M, Torres J
Cell Tissue Res. 1978 Dec 28;195(2):239-50. doi: 10.1007/BF00236722.
Guanethidine-induced sympathectomy in the rat during the neonatal period (injection of 20 microgram/g body weight every 48 h from day of birth until day 14) produces an absolute reduction in the number of sympathetic ganglion cells, but no significant alteration of body weight. Superior cervical ganglia show 79.8% fewer cell bodies at 15 days and 92.3% at 45 days; coeliac ganglia exhibit an 81.0% reduction at 15 days and 89.6% at 45 days in guanethidine-treated rats as compared to normal controls. The sympathetic ganglion cells that remain after treatment have an abnormal morphological appearance with distended mitochondria and depletion of endoplasmic reticulum. Sympathectomy produces a prolongation of the generation cycle time (Tc) as measured by the colchicine-induced mitotic arrest technique, and a decrease in labelling, mitotic, and migration indices. In addition, sympathectomy suppresses the amplitude of the circadian rhythm in mitotic activity. The general suppression of this activity in the intestinal epithelium is more pronounced in the jejunum and ileum than in the duodenum. Variation in the effectiveness of sympathectomy on the inhibition of intestinal cell proliferation may be related to segmental differences in cell proliferation, to segmental differences in innervation, and/or to segmental variation in the effectiveness of guanethidine.
新生期大鼠经胍乙啶诱导的交感神经切除术（从出生日至第14天，每48小时注射20微克/克体重）可使交感神经节细胞数量绝对减少，但体重无显著改变。颈上神经节在15天时细胞体减少79.8%，45天时减少92.3%；与正常对照组相比，胍乙啶处理的大鼠腹腔神经节在15天时减少81.0%，45天时减少89.6%。处理后残留的交感神经节细胞形态异常，线粒体肿胀，内质网减少。通过秋水仙碱诱导的有丝分裂阻滞技术测量，交感神经切除术可使细胞周期时间（Tc）延长，并使标记指数、有丝分裂指数和迁移指数降低。此外，交感神经切除术可抑制有丝分裂活动的昼夜节律幅度。肠道上皮中这种活动的总体抑制在空肠和回肠比在十二指肠更明显。交感神经切除术对肠道细胞增殖抑制作用的有效性差异可能与细胞增殖的节段差异、神经支配的节段差异和/或胍乙啶有效性的节段差异有关。
