Rubányi G, Kovách A G
Circ Shock. 1980;7(2):121-7.
Rat hearts were isolated from two groups of animals: I, Control (C); and II, Anesthetized with sodium pentobarbital (Nembutal) (P) 5 mg/100 g body weight prior to decapitation and perfused by modified Langendorff-technique. Left ventricular mechanical performance (LVSP, LVPD, dp/dtmax) and O2 consumption of P hearts was identical to that of C hearts. P hearts exhibited significantly lower coronary resistance and higher spontaneous heart rate. During electrical pacing (240--480/min) C and P hearts showed similar increase of mechanical performance. In sharp contrast to C hearts, P hearts were completely insensitive to threefold elevation of perfusate Ca2+ (from 1.3 to 3.9 mM). Experimental results suggest that pentobarbital anesthesia exerts a long-lasting effect on the isolated myocardium, which should be considered if tissues are isolated from animals treated by this drug.
I组，对照组（C）；II组，在断头前用5mg/100g体重的戊巴比妥钠（戊巴比妥）（P）麻醉，并采用改良的Langendorff技术进行灌注。P组心脏的左心室机械性能（LVSP、LVPD、dp/dtmax）和耗氧量与C组心脏相同。P组心脏的冠状动脉阻力显著较低，自发心率较高。在电起搏（240 - 480次/分钟）期间，C组和P组心脏的机械性能表现出相似的增加。与C组心脏形成鲜明对比的是，P组心脏对灌注液Ca2+浓度三倍升高（从1.3 mM升至3.9 mM）完全不敏感。实验结果表明，戊巴比妥麻醉对离体心肌有持久影响，如果从用该药物处理过的动物中分离组织，应考虑这一点。
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