Migration of 15 micron microspheres from infarcted myocardium.

The fate of 15 micrometers microspheres in ischaemic myocardial tissue was investigated. The spheres were delivered to the left atrium before acute coronary artery ligation. 10 h later the myocardium was examined for its contents of preocclusion spheres, as well as spheres delivered immediately before sacrifice. The ischaemic tissue contained 11 to 60% less preocclusion spheres, compared with nonischaemic tissue in the same hearts. On average, 26% of preocclusion spheres were lost during the 10 h period. Negligible amounts of the lost spheres could be retrieved from cardiac lymph nodes in the mediastinum. Most likely, the spheres migrated through venous pathways; a substantial number of preocclusion spheres were present in the lungs after 10 h, the bulk of which had originated from other organs than the infarct. These results quantify an important limitation to the use of microsphere distribution as a method for tissue blood flow measurements in infarcted myocardium when the period of coronary artery occlusion exceeds a few hours.