Bakkerud K G, Nissen P
Biochim Biophys Acta. 1980 Jul 16;600(1):205-11. doi: 10.1016/0005-2736(80)90425-3.
The ability of bacteria to cause rapid uptake of choline sulfate in plants, i.e., effectiveness, was studied using Pseudomonas tolaasii and excised roots of barley (Hordeum vulgare L.). Once effective, bacteria remained so after being killed by treatments which cause little damage to their outer structure. However, effectiveness was destroyed by disruption of the cell wall, protein reagents, a mild heat treatment or removal of Mg2+. Effective bacteria adsorbed choline sulfate. This adsorption had characteristics similar to those of bacterial effectiveness (magnesium requirement, high substrate specificity). These results indicate that a proteinaceous structure on the bacterial surface binds and, somehow, transfers choline sulfate to the plant.
利用托拉氏假单胞菌和大麦（Hordeum vulgare L.）离体根，研究了细菌促使植物快速吸收硫酸胆碱的能力，即有效性。一旦具有有效性，细菌在经对外层结构几乎无损伤的处理而被杀死后仍保持这种能力。然而，细胞壁破坏、蛋白质试剂、温和热处理或去除Mg2+会破坏其有效性。有效的细菌吸附硫酸胆碱。这种吸附具有与细菌有效性相似的特征（对镁的需求、高底物特异性）。这些结果表明，细菌表面的一种蛋白质结构结合硫酸胆碱，并以某种方式将其转移至植物。
