Addison W C
J Anat. 1980 May;130(Pt 3):479-86.
Twelve kittens aged 18 weeks each received a single injection via a femoral vein; eight were given 4 USP units PTE per kg body weight and four were given an equivalent amount of normal saline. Four PTE and four saline (control) animals were killed after 1 hour and four PTE kittens were killed after 6 hours. Fresh imprints of femoral metaphyseal bone were stained by histochemical methods for succinate, malate, betahydroxy butyrate and glutamate dehydrogenases. These preparations were similar and the unstained nuclei contrasted sharply with the background stained cytoplasm, making possible accurate nucleus counts. Cumulative frequency distribution for control and PTE 1 hour osteoclasts samples were similar asymmetric curves with a positive skew with no statistically significant difference between them. chi2 analyses did, however reveal a statistically highly significant (P < 0.0001) difference between PTE 6 hours osteoclasts and both untreated and PTE 1 hour samples. The arithmetic mean and median numbers of nuclei increased 6 hours after administration of PTE. The results suggest that in kittens an early response of osteolcasts to PTE in vivo is an increase in the numbers of contained nuclei.
12只18周龄的小猫通过股静脉接受单次注射；8只每千克体重注射4 USP单位的PTE，4只注射等量的生理盐水。4只注射PTE和4只注射生理盐水（对照）的动物在1小时后处死，4只注射PTE的小猫在6小时后处死。采用组织化学方法对股骨干骺端新鲜印片进行琥珀酸脱氢酶、苹果酸脱氢酶、β-羟基丁酸脱氢酶和谷氨酸脱氢酶染色。这些标本相似，未染色的细胞核与染色的细胞质背景形成鲜明对比，从而能够准确计数细胞核。对照和PTE 1小时破骨细胞样本的累积频率分布是相似的不对称曲线，呈正偏态，两者之间无统计学显著差异。然而，chi2分析显示，PTE 6小时破骨细胞与未处理样本及PTE 1小时样本之间存在统计学高度显著差异（P < 0.0001）。注射PTE 6小时后，细胞核的算术平均值和中位数增加。结果表明，在小猫体内，破骨细胞对PTE的早期反应是所含细胞核数量增加。
