通过放射免疫分析法对胆汁中前列腺素F化合物进行鉴定和定量分析。

Identification and quantitation by radioimmunoassay of prostaglandin F compounds in bile.

作者信息

Deshpande Y G, Kaminski D L

出版信息

Prostaglandins. 1980 Aug;20(2):367-72. doi: 10.1016/s0090-6980(80)80053-0.

Abstract

Separation and identification of prostaglandins in canine bile was performed by extraction and thin layer chromatography. The system provided tentative identification of the prostaglandin F compounds as the major prostaglandin subgroup present in bile. The prostaglandin was subsequently purified on silicic acid columns and quantitated by radioimmunoassay with tritiated PGF2 alpha and anti PGF2 alpha antibody employing the double antibody technique. Basal levels in hepatic bile were found to be 1028 +/- 98 pg/ml.

摘要

通过萃取和薄层色谱法对犬胆汁中的前列腺素进行分离和鉴定。该系统初步鉴定出前列腺素F化合物是胆汁中存在的主要前列腺素亚组。随后，前列腺素在硅酸柱上进行纯化，并采用双抗体技术，用氚标记的前列腺素F2α和抗前列腺素F2α抗体通过放射免疫测定法定量。发现肝胆汁中的基础水平为1028±98 pg/ml。