Hochman J, Katz A, Levy E, Eshel S
Cell Biol Int Rep. 1980 Oct;4(10):953-9. doi: 10.1016/0309-1651(80)90198-8.
A new variant subline was selected from S49 mouse lymphoma cells grown in suspension culture. These cells, designated S49-Adh, demonstrate increased adhesiveness to their substratum. This, in turn, allows them to grow as a monolayer. Once a dense monolayer is formed, daughter cells develop as a suspension borne population while still retaining their substrate adhesiveness. Cell substrate adhesiveness is a stable genetic trait insofar as submitting S49-Adh cells to both extended periods of in vitro cultivation in culture medium, or in vivo selection through passage in nude mice didn't change their substrate-adhesive phenotype.