Shcherbakova I Iu, Shutilova N I, Kadoshnikova I G, Giller Iu E
Mol Biol (Mosk). 1980 Jul-Aug;14(4):881-90.
The effect of specific inhibitors of translation in chloroplasts (chloramphenicol) and in cytoplasm (cycloheximide) on the formation of pigment-protein-lipid complexes of photosynthetic membranes, on the chlorophyll state in chloroplasts and isolated membrane complexes had been studied. It is proved that the inhibition of translation blocks chlorophyll incorporation only into the complexes of reaction centres of photosystems without any change of the light-harvesting complex. The action of inhibitors leads to the disappearance of long-wave native forms of pigment in the complexes of reaction centres, which are characteristic for them. But the action of inhibitors does not effect the formation of non-specific short-wave forms which are present in all types of membrane complexes. Chloramphenicol proved to be more active in such processes than cycloheximide. On the basis of the data on localization of biosynthesis of polypeptide components of plastid membranes we suppose that during biogenesis of the photosynthetic apparatus the conditions for self-assembly of non-specific native forms of chlorophyll in light-harvesting complexes are made as the result of polymerazation of the main membrane polypeptides which are synthesized in the cytoplasms. Minor polypeptides of plastid (photosystems 1 and 2) and cytoplasmic (photosystem 1) orgin are necessary for self-assembly of the dense units of pigment in reaction centre complexes.
研究了叶绿体中翻译的特异性抑制剂（氯霉素）和细胞质中翻译的特异性抑制剂（环己酰亚胺）对光合膜色素 - 蛋白质 - 脂质复合物形成、叶绿体及分离的膜复合物中叶绿素状态的影响。结果证明，翻译抑制仅阻止叶绿素掺入光系统反应中心复合物，而光捕获复合物无任何变化。抑制剂的作用导致反应中心复合物中具有特征性的长波天然色素形式消失。但抑制剂的作用不影响所有类型膜复合物中存在的非特异性短波形式的形成。氯霉素在这些过程中比环己酰亚胺更具活性。基于质体膜多肽成分生物合成定位的数据，我们推测在光合装置生物发生过程中，光捕获复合物中非特异性天然叶绿素形式自我组装的条件是由在细胞质中合成的主要膜多肽聚合产生的。质体（光系统1和2）和细胞质（光系统1）来源的小多肽对于反应中心复合物中色素密集单元的自我组装是必需的。
