Znidarić D, Lui A, Kalafatić M
Z Mikrosk Anat Forsch. 1980;94(1):179-87.
Budles and one-budded hydras were exposed to action of antimite (N-methyl-bis-chlorethylamine hydrochloride, Pliva, Zagreb) in 7 mg/150, 7 mg/200 and 7 mg/250 ml concentrations. All these concentrations caused exterior morphological and cytological changes of the hydra body. In the ectodermal layer interstitial cells gradually disappeared, and then followed enidoblasts and enids. The discussion deals with interstitial cells in a normal untreated animal and possible differentiation of these cells in enidoblasts and enids after the action of antimite. Antimite prevents mitosis, but it does not stop differentiation of some of the cells into other cell types. In the gastrodermal layer the greatest changes which are caused by this cytostatic can be seen on zymogen cells. Their shape, cell structure and position in the gastroderm is changed. The results show that zymogen cells after the antimite action have a limited ability of dedifferentiation into gastrodermal interstitial cells, and that their ability to differentiate into mucous cells lingers a longer time. This depends upon their age. Only the hydras which were acted upon by such a dose of antimite that does not prevent division at last some of the zymogen cells can survive and wholly continue their development.
将芽体和单芽水螅暴露于不同浓度的抗瘤药（N - 甲基 - 双 - 氯乙胺盐酸盐，普利瓦公司，萨格勒布）中，浓度分别为7毫克/150毫升、7毫克/200毫升和7毫克/250毫升。所有这些浓度都会引起水螅身体的外部形态和细胞学变化。在外胚层中，间质细胞逐渐消失，接着是刺细胞母细胞和刺细胞。讨论涉及正常未处理动物中的间质细胞以及抗瘤药作用后这些细胞向刺细胞母细胞和刺细胞的可能分化。抗瘤药阻止有丝分裂，但并不阻止一些细胞分化为其他细胞类型。在内胚层中，这种细胞抑制剂引起的最大变化可见于酶原细胞。它们的形状、细胞结构以及在内胚层中的位置都发生了改变。结果表明，抗瘤药作用后的酶原细胞向胃层间质细胞去分化的能力有限，并且它们分化为黏液细胞的能力持续时间更长。这取决于它们的年龄。只有受到这种剂量抗瘤药作用且最终不阻止一些酶原细胞分裂的水螅才能存活并完全继续其发育。
