皮质醇尿酸性代谢产物的气相色谱和质谱分析。

Gas chromatographic and mass spectrometric analysis of urinary acidic metabolites of cortisol.

作者信息

Shackleton C H, Roitman E, Monder C, Bradlow H L

出版信息

Steroids. 1980 Sep;36(3):289-98. doi: 10.1016/0039-128x(80)90003-3.

Abstract

A method is described suitable for the analysis of the urinary acidic metabolites of cortisol which are amongst the major metabolites of this hormone (5-25% of secretion). Following hydrolysis of the urinary glucuronide conjugates and extraction of the freed steroids, methyl ester-trimethylsilyl ethers were prepared for gas chromatographic analysis. This analysis was carried out on open tubular columns coated with Carbowax stationary phase. The polar phase column permitted the complete resolution of the four acidic metabolites: alpha-cortolonic, beta-cortolonic, alpha-cortolic and beta-cortolic acids.

摘要

本文描述了一种适用于分析皮质醇尿液酸性代谢物的方法，这些代谢物是该激素的主要代谢产物（占分泌量的5-25%）。在对尿液葡萄糖醛酸结合物进行水解并提取游离类固醇后，制备甲酯-三甲基硅醚用于气相色谱分析。该分析在涂有Carbowax固定相的开管柱上进行。极性相柱能够完全分离四种酸性代谢物：α-皮质酮酸、β-皮质酮酸、α-皮质醇酸和β-皮质醇酸。