母鸡垂体和下丘脑组织中的一种细胞质孕酮受体。

A cytoplasmic progesterone receptor in hen pituitary and hypothalamic tissues.

作者信息

Kawashima M, Kamiyoshi M, Tanaka K

出版信息

Endocrinology. 1978 Apr;102(4):1207-13. doi: 10.1210/endo-102-4-1207.

Abstract

The cytosol fraction of the anterior lobe of the pituitary in laying White Leghorn hens was shown to possess a progesterone-binding component which perferentially bound progesterone. A similar component was also found in the cytosol of the hypothalamus and of the oviduct magnum. The dissociation constant (Kd) at equilibrium was determined as 1.68 +/- 0.10 (SE) X 10(-10) M in the pituitary, 3.29 +/- 0.10 x 10(-10) M in the hypothalamus, and 3.02 +/- 0.40 x 10(-10) M in the oviduct magnum, whereas the number of binding sites/mg cytosol protein was 5.99 +/- 0.084 (SE) X 10(-13) mol in the pituitary, 0.43 +/- 0.003 x 10(-13) mol in the hypothalamus, and 2.88 +/- 0.114 x 10(-13) mol in the oviduct magnum. The results suggest that a cytoplasmic progesterone receptor is present in the pituitary and hypothalamic tissue, as well as in the oviduct magnum, of the hen.

摘要

结果表明，产蛋白来航鸡垂体前叶的胞质部分含有一种能优先结合孕酮的孕酮结合成分。在下丘脑和输卵管峡部的胞质中也发现了类似的成分。垂体中平衡解离常数（Kd）测定为1.68±0.10（标准误）×10⁻¹⁰ M，下丘脑中为3.29±0.10×10⁻¹⁰ M，输卵管峡部中为3.02±0.40×10⁻¹⁰ M，而每毫克胞质蛋白的结合位点数在垂体中为5.99±0.084（标准误）×10⁻¹³ mol，下丘脑中为0.43±0.003×10⁻¹³ mol，输卵管峡部中为2.88±0.114×10⁻¹³ mol。结果提示，母鸡的垂体、下丘脑组织以及输卵管峡部中存在一种细胞质孕酮受体。