Herzberg M, Breitbart H
Mol Biol Rep. 1980 Oct 31;6(3):163-7. doi: 10.1007/BF00775411.
In this paper we show that ribosomes isolated from cells inhibited for protein synthesis by the antibiotic ionophore Valinomycin can still support incorporation of [14C]-leucine into polypeptides in a cell-free system. The extent of this functional integrity depends upon the concentration of Valinomycin used and whether it acted as an ionophore or not. We demonstrate that the antibiotic acts on protein synthesis at the level of elongation and has no action either at the initiation level or as activating an RNase. Moreover, we show that it can inhibit protein synthesis at concentrations where its action as an ionophore cannot be detected.
在本文中，我们表明，从被抗生素离子载体缬氨霉素抑制蛋白质合成的细胞中分离出的核糖体，在无细胞系统中仍能支持将[14C] - 亮氨酸掺入多肽中。这种功能完整性的程度取决于所用缬氨霉素的浓度以及它是否作为离子载体起作用。我们证明，这种抗生素在延伸水平上作用于蛋白质合成，在起始水平或作为激活核糖核酸酶方面均无作用。此外，我们表明，在无法检测到其作为离子载体作用的浓度下，它就能抑制蛋白质合成。
