Meredith S A, Ganschow R E
Genetics. 1978 Dec;90(4):725-34. doi: 10.1093/genetics/90.4.725.
A difference in the heat-inactivation kinetics between the beta-glucuronidases of C3HeB/FeJ and C57B1/6J mice was utilized to assess the mode of action of a temporal genetic element in controlling the expression of the beta-glucuronidase structural gene Gus. The heat-inactivation kinetics of liver and kidney beta-glucuronidase from F1 C3HeB/FeJ X C57B1/6J animals were intermediate with respect to the parental enzyme patterns, suggesting that equal concentrations of the two allelic products were present in beta-glucuronidase tetramers of F1 progeny. beta-glucuronidase heteropolymers assembled in vivo under conditions where equal concentrations of the two structural alleles of the enzyme were known to be present also exhibited intermediate heat-inactivation kinetics. These observations are consistent with a trans mode of action of a genetic element that controls the rate of murine beta-glucuronidase synthesis.
利用C3HeB/FeJ和C57B1/6J小鼠β-葡萄糖醛酸酶之间热失活动力学的差异,来评估一个时间遗传元件在控制β-葡萄糖醛酸酶结构基因Gus表达中的作用模式。F1代C3HeB/FeJ×C57B1/6J动物肝脏和肾脏β-葡萄糖醛酸酶的热失活动力学相对于亲本酶模式处于中间状态,这表明在F1代子代的β-葡萄糖醛酸酶四聚体中存在等量浓度的两种等位基因产物。在已知存在等量浓度的该酶两种结构等位基因的条件下,体内组装的β-葡萄糖醛酸酶异源聚合物也表现出中间热失活动力学。这些观察结果与控制小鼠β-葡萄糖醛酸酶合成速率的遗传元件的反式作用模式一致。