用于DNA测定的二苯胺反应的改进应用。
Improved application of the diphenylamine reaction for the determination of DNA.
作者信息
Jalkanen M, Larjava H, Turakainen H, Tammi M
出版信息
J Biochem Biophys Methods. 1980 Oct;3(4):195-9. doi: 10.1016/0165-022x(80)90057-3.
PMID:7451812
Abstract
An improved procedure is described for the separation and quantitation of DNA from biological samples. Following papain digestion, DNA was separated from other cell material with CdCl2 precipitation onto a glass-fiber filter and quantitated easily by the modified diphenylamine reaction.
摘要
本文描述了一种改进的从生物样品中分离和定量DNA的方法。木瓜蛋白酶消化后，通过氯化镉沉淀将DNA与其他细胞物质分离，沉淀在玻璃纤维滤膜上，然后通过改良的二苯胺反应轻松进行定量。
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