Knook D L, Sleyster E C, Teutsch H F
Histochemistry. 1980;69(2):211-6. doi: 10.1007/BF00533138.
Sinusoidal cells in the rat liver react intensively for G6DPH activity after appropriate incubation (Rieder et al. 1978). After isolation and purification of the sinusoidal Kupffer and endothelial cells, it was demonstrated that Kupffer cells exhibit a 5-8 times higher G6PDH activity on a per cell basis by comparison with endothelial cells, while the specific G6PDH activity was 3-4 times higher in Kupffer cells. The Kupffer cells can be divided into two groups which differ significantly in G6PDH activity calculated on a per cell basis. In histochemical studies, G6PDH can be used as a marker for Kupffer cell identification.
经过适当孵育后,大鼠肝脏中的窦状隙细胞对葡萄糖-6-磷酸脱氢酶(G6DPH)活性反应强烈(里德等人,1978年)。在分离和纯化窦状隙库普弗细胞和内皮细胞后,结果表明,与内皮细胞相比,库普弗细胞以单个细胞为基础的G6PDH活性高5-8倍,而库普弗细胞中的G6PDH比活性高3-4倍。库普弗细胞可分为两组,以单个细胞为基础计算的G6PDH活性有显著差异。在组织化学研究中,G6PDH可作为鉴定库普弗细胞的标志物。