LacI和LacZ转基因小鼠突变试验的比较：一项欧盟资助的实验室间研究。

Comparison of lacI and lacZ transgenic mouse mutation assays: an EU-sponsored interlaboratory study.

作者信息

Tinwell H, Liegibel U, Krebs O, Schmezer P, Favor J, Ashby J

机构信息

Zeneca Central Toxicology Laboratory, Macclesfield, Cheshire, UK.

出版信息

Mutat Res. 1995 Oct;335(2):185-90. doi: 10.1016/0165-1161(95)90054-3.

DOI:10.1016/0165-1161(95)90054-3

PMID:7477049

Abstract

As part of an EU Environment Programme sponsored study, the performance of two transgenic mouse mutation assays has been evaluated in three laboratories using common liver samples. The systems studied were the lacI-(Big Blue) mutation assay, and the GalE- positive selection modified lacZ- (Muta Mouse) assay. The liver samples compared were derived from mice treated with either saline or dimethylnitrosamine (DMN; 14 day recovery). Each assay gave an increased mutation frequency (MF) for the DMN-treated livers when compared to the saline control MFs. Sources of variability in the assays are discussed, and it is concluded that whole liver should be homogenised before DNA extraction, and that concurrent controls should be processed in parallel with test samples.

摘要

作为欧盟环境计划资助研究的一部分，在三个实验室中使用常见肝脏样本对两种转基因小鼠突变试验的性能进行了评估。所研究的系统是lacI-（大蓝）突变试验和GalE阳性选择修饰的lacZ-（突变小鼠）试验。所比较的肝脏样本来自用生理盐水或二甲基亚硝胺（DMN；14天恢复期）处理的小鼠。与生理盐水对照的突变频率相比，每种试验对经DMN处理的肝脏均给出了更高的突变频率（MF）。讨论了试验中的变异性来源，并得出结论，在提取DNA之前应将整个肝脏匀浆，并且应与测试样品并行处理同期对照。