Vischjager M, Van Gulik T M, Pfaffendorf M, Van Zwieten P A, Van Marle J, Kromhout J G, Klopper P J, Jacobs M J
Department of Surgery, University of Amsterdam, The Netherlands.
Eur J Vasc Endovasc Surg. 1995 Nov;10(4):431-9. doi: 10.1016/s1078-5884(05)80165-0.
To assess the function of arterial grafts after prolonged preservation in the University of Wisconsin solution (UW), in vitro and in vivo.
Carotid arteries were harvested from dogs and stored for 1-21 days at 4 degrees C in UW (n = 10) or in PBS (0.9% NaCl, pH 7.4), (PBS) (n = 10). Slices were examined by lightmicroscopy (LM) and scanning electron microscopy (SEM). For viability testing, specimens were connected to an isometric force transducer (2 x n = 9). Contractile and relaxation responses were examined by adding phenylephrine (200 microM) and metacholine (200 microM), respectively. For in vivo studies (n = 41), 2.5cm carotid artery segments were implanted or orthotopically, as autografts and allografts, after 14 days of storage in UW or in PBS. Autologous veins were used as controls. After 28 days or 56 days, arteriography was performed and the grafts were excised for LM and SEM.
The arterial endothelial layer remained intact after up to 14 days of storage in UW. In PBS, the endothelium was lost after 3 days. The functional response after 14 days storage in UW was approximately 50% vs. 0% after 14 days in PBS. In the autografts, total patencies (28 days + 56 days) were 100% (8/8) and 63% (5/8) for UW and PBS stored grafts, respectively. In the allografts, the UW and PBS preserved grafts showed total patencies of 86% (12/14) and 83% (5/6), respectively. Microscopically, the allografts showed fibrotic degeneration.
Arteries are well preserved in UW up to 14 days of storage. Arterial autografts preserved in UW showed good patency and better integrity of the vessel wall after implantation, than grafts stored in PBS or allografts (without immunosuppressive therapy).
在体外和体内评估威斯康星大学溶液（UW）中长期保存后的动脉移植物功能。
从犬身上获取颈动脉，在4℃下于UW（n = 10）或磷酸盐缓冲液（PBS，0.9% NaCl，pH 7.4）（n = 10）中保存1 - 21天。切片通过光学显微镜（LM）和扫描电子显微镜（SEM）检查。进行活力测试时，将标本连接到等长力传感器（2×n = 9）。分别加入去氧肾上腺素（200μM）和乙酰甲胆碱（200μM）检查收缩和舒张反应。对于体内研究（n = 41），在UW或PBS中保存14天后，将2.5cm颈动脉段作为自体移植物和同种异体移植物原位植入。自体静脉用作对照。28天或56天后，进行血管造影，然后切除移植物进行LM和SEM检查。
在UW中保存长达14天后，动脉内皮层保持完整。在PBS中，3天后内皮消失。在UW中保存14天后的功能反应约为50%，而在PBS中保存14天后为0%。在自体移植物中，UW保存的移植物和PBS保存的移植物的总通畅率（28天 + 56天）分别为100%（8/8）和63%（5/8）。在同种异体移植物中，UW保存的移植物和PBS保存的移植物的总通畅率分别为86%（12/14）和83%（5/6）。显微镜下，同种异体移植物显示出纤维化变性。
动脉在UW中保存长达14天能得到良好保存。与保存在PBS中的移植物或同种异体移植物（无免疫抑制治疗）相比，UW保存的动脉自体移植物植入后显示出良好的通畅率和更好的血管壁完整性。
