从Tamm-Horsfall糖蛋白中获得的一种92 kDa人免疫刺激糖蛋白的纯化

Purification of a 92 kDa human immunostimulating glycoprotein obtained from the Tamm-Horsfall glycoprotein.

作者信息

Fontan E, Jusforgues-Saklani H, Briend E, Fauve R M

机构信息

Unite d'Immunophysiologie Cellulaire, Institut Pasteur, Paris, France.

出版信息

J Immunol Methods. 1995 Nov 16;187(1):81-4. doi: 10.1016/0022-1759(95)00169-b.

DOI:10.1016/0022-1759(95)00169-b

PMID:7490460

Abstract

A purification method for a human urinary glycoprotein (HGP92) dissociated from Tamm Horsfall protein (THP) is described. Tamm-Horsfall protein, obtained by salt precipitations, was again precipitated in presence of monovalent ions. In these conditions, Tamm-Horsfall protein displayed a tendency to form a gel. After ultracentrifugation, HGP92, which was trapped in the gel, was dissociated from Tamm-Horsfall protein and found in the supernatant. The final step of purification of HGP92 was chromatography on a DEAE Affigel blue column. Injected intravenously, HGP92, but not THP, protected mice against a lethal inoculum of Listeria monocytogenes. This procedure has the advantage of being easy to perform, and enables preparation of large amounts of HGP92. These results suggest that the previously described 'immunostimulating' properties of Tamm-Horsfall protein were, in fact, a consequence of its contamination by HGP92.

摘要

本文描述了一种从Tamm-Horsfall蛋白（THP）中分离出的人尿糖蛋白（HGP92）的纯化方法。通过盐沉淀获得的Tamm-Horsfall蛋白，在一价离子存在的情况下再次沉淀。在这些条件下，Tamm-Horsfall蛋白呈现出形成凝胶的趋势。超速离心后，被困在凝胶中的HGP92从Tamm-Horsfall蛋白中解离出来并存在于上清液中。HGP92纯化的最后一步是在DEAE Affigel蓝柱上进行层析。静脉注射HGP92而非THP可保护小鼠免受致死剂量单核细胞增生李斯特菌的感染。该方法具有易于操作的优点，并且能够制备大量的HGP92。这些结果表明，先前描述的Tamm-Horsfall蛋白的“免疫刺激”特性实际上是其被HGP92污染的结果。