Kikuchi Y, Kobayashi E, Nishizawa M, Hamazaki S, Okada S, Hino O
Department of Experimental Pathology, Cancer Institute, Tokyo.
Jpn J Cancer Res. 1995 Oct;86(10):905-9. doi: 10.1111/j.1349-7006.1995.tb02999.x.
Recently, von Hippel-Lindau (VHL) gene mutations were detected in non-inherited, sporadic human renal cell carcinomas (RCs) at a high frequency. In order to determine whether or not the VHL gene is also a critical gene in rat RCs, we cloned and sequenced the rat homologue of human VHL gene and searched for mutations of the VHL gene in rat RCs. Mutations in the VHL gene were not detected in spontaneous RCs of the Eker rat model or in ferric nitrilotriacetate-induced rat RCs using the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) method. These data indicate that mutation of the VHL tumor suppressor gene is not an event in rat renal carcinogenesis, at least in our present systems.
最近，在非遗传性散发性人类肾细胞癌（RC）中高频检测到冯·希佩尔－林道（VHL）基因突变。为了确定VHL基因在大鼠RC中是否也是关键基因，我们克隆并测序了人类VHL基因的大鼠同源物，并在大鼠RC中寻找VHL基因的突变。使用聚合酶链反应－单链构象多态性（PCR－SSCP）方法，在Eker大鼠模型的自发性RC或次氮基三乙酸铁诱导的大鼠RC中未检测到VHL基因突变。这些数据表明，至少在我们目前的系统中，VHL肿瘤抑制基因突变不是大鼠肾癌发生过程中的一个事件。
