一种新型肌球蛋白轻链激酶在胚胎组织和培养细胞中的表达。
Expression of a novel myosin light chain kinase in embryonic tissues and cultured cells.
作者信息
Gallagher P J, Garcia J G, Herring B P
机构信息
Department of Physiology, Indiana University School of Medicine, Indianapolis 46202-5120, USA.
出版信息
J Biol Chem. 1995 Dec 8;270(49):29090-5. doi: 10.1074/jbc.270.49.29090.
Abstract
A novel, 208-kDa myosin light chain kinase (MLCK) distinct from smooth muscle and non-muscle MLCK has been identified by cross-reaction to two antibodies raised against smooth muscle MLCK. Additional antibodies directed against the amino and carboxyl termini of the smooth muscle MLCK do not react with the 208-kDa MLCK, suggesting these regions are distinct. 208-kDa MLCK phosphorylates 20-kDa myosin light chains in a Ca2+/calmodulin-dependent manner, consistent with it being a member of the MLCK family. Expression of 208-kDa MLCK and smooth muscle MLCK appears to be inversely regulated, with 208-kDa MLCK being most abundant during early development and declining at birth. In contrast, expression of smooth muscle MLCK is relatively low early during development and increases to become the predominant MLCK detected in all adult smooth and non-muscle tissues. The developmental expression pattern of the 208-kDa MLCK suggests this form be named, embryonic MLCK.
摘要
通过与针对平滑肌肌球蛋白轻链激酶（MLCK）产生的两种抗体发生交叉反应，已鉴定出一种与平滑肌和非肌肉MLCK不同的新型208 kDa肌球蛋白轻链激酶。针对平滑肌MLCK氨基和羧基末端的其他抗体不与208 kDa MLCK反应，表明这些区域不同。208 kDa MLCK以Ca2+/钙调蛋白依赖性方式磷酸化20 kDa肌球蛋白轻链，这与其作为MLCK家族成员一致。208 kDa MLCK和平滑肌MLCK的表达似乎受到反向调节，208 kDa MLCK在早期发育期间最为丰富，出生时下降。相反，平滑肌MLCK的表达在发育早期相对较低，并增加成为在所有成年平滑肌和非肌肉组织中检测到的主要MLCK。208 kDa MLCK的发育表达模式表明这种形式应命名为胚胎MLCK。
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