体外聚（ADP - 核糖）周转系统中的平衡模型

Equilibrium model in an in vitro poly(ADP-ribose) turnover system.

作者信息

Lagueux J, Ménard L, Candas B, Brochu G, Potvin F, Verreault A, Cook P F, Poirier G G

机构信息

Molecular Endocrinology Laboratory, Faculty of Medicine, Laval University, Sainte-Foy, Québec, Canada.

出版信息

Biochim Biophys Acta. 1995 Nov 7;1264(2):201-8. doi: 10.1016/0167-4781(95)00143-5.

DOI:10.1016/0167-4781(95)00143-5

PMID:7495864

Abstract

Poly(ADP-ribose) metabolism plays an important role in numerous DNA-related functions. This homopolymer is synthesized by poly(ADP-ribose) polymerase and is degraded mainly by the poly(ADP-ribose) glycohydrolase. The activities of these two enzymes in the nucleus are closely coordinated. To better understand the interactions between these enzymes, we designed an in vitro system in which both enzymes are present at the same time. In this work, we report a model describing the synthesis and degradation of the poly(ADP-ribose) in turnover conditions. Because the half-life of the polymer in the cell is close to 1 min, we studied the very early kinetic interactions of these two enzymes.

摘要

聚（ADP-核糖）代谢在众多与DNA相关的功能中发挥着重要作用。这种同聚物由聚（ADP-核糖）聚合酶合成，主要由聚（ADP-核糖）糖苷水解酶降解。这两种酶在细胞核中的活性密切协调。为了更好地理解这些酶之间的相互作用，我们设计了一个体外系统，其中两种酶同时存在。在这项工作中，我们报告了一个描述聚（ADP-核糖）在周转条件下合成和降解的模型。由于该聚合物在细胞中的半衰期接近1分钟，我们研究了这两种酶非常早期的动力学相互作用。