Kwittken P L, Pawlowski N A, Sweinberg S K, Douglas S D, Campbell D E
Division of Allergy, Immunology, and Infectious Diseases, Children's Hospital of Philadelphia, Pennsylvania 19104, USA.
Clin Diagn Lab Immunol. 1994 Mar;1(2):197-201. doi: 10.1128/cdli.1.2.197-201.1994.
Immediate hypersensitivity to natural latex (NL) occurs in sensitized individuals after repeated exposure to products or devices containing NL components. Since allergic reactions to NL proteins are quite frequent and may be quite serious, diagnostic assays are needed to identify individuals at risk. A number of latex proteins have been considered the major antigens, but they have been incompletely characterized. There is no standard material available for skin testing. In vitro diagnostic tests, such as the radioallergosorbent test (RAST), are time consuming and their sensitivity and specificity remain to be proven. We have developed a rapid microsphere-based, fluorescence-activated flow cytometry assay for the measurement of NL protein-specific human immunoglobulin E and have compared it with both the enzyme-linked immunosorbent assay and radioallergosorbent test methods. By using the total purified NL protein fraction isolated from raw ammoniated NL sap as the antigen, the flow cytometry assay was both sensitive and specific for the detection of NL protein-specific human immunoglobulin E in the sera of sensitized pediatric patients.
对天然乳胶（NL）的速发型超敏反应发生在致敏个体反复接触含有NL成分的产品或装置之后。由于对NL蛋白的过敏反应相当常见且可能相当严重，因此需要诊断检测来识别有风险的个体。多种乳胶蛋白已被视为主要抗原，但它们的特征尚未完全明确。目前尚无用于皮肤试验的标准材料。体外诊断试验，如放射变应原吸附试验（RAST），耗时较长，其敏感性和特异性仍有待证实。我们开发了一种基于微球的快速荧光激活流式细胞术检测方法，用于检测NL蛋白特异性人免疫球蛋白E，并将其与酶联免疫吸附测定法和放射变应原吸附试验方法进行了比较。通过使用从生氨化NL树液中分离得到的总纯化NL蛋白组分作为抗原，流式细胞术检测对于检测致敏儿科患者血清中的NL蛋白特异性人免疫球蛋白E既敏感又特异。
