Hamm R J, Pike B R, Temple M D, O'Dell D M, Lyeth B G
Department of Psychology, Virginia Commonwealth University/Medical College of Virginia, Richmond 23284-2018, USA.
Exp Neurol. 1995 Dec;136(2):143-8. doi: 10.1006/exnr.1995.1091.
The purpose of this experiment was to examine the consequences of postinjury seizures on cognitive performance after experimental traumatic brain injury (TBI). Rats either were injured at a moderate (2.1 atm) level of central fluid percussion TBI (n = 16) or were surgically prepared but did not receive a fluid pulse (sham-injured control, n = 16). Beginning 24 h after TBI, injured animals were injected (ip) once daily (Days 1-24 postinjury) with either saline (n = 8) or 25 mg/kg pentylenetetrazol (PTZ) (n = 8). Sham-injured rats were injected with an equal volume of saline (n = 8) or PTZ (n = 8). In both injured and sham-injured animals, daily injections of PTZ resulted in an increase in the severity of behavioral seizures over days. On Days 25-29 after injury or sham injury, all animals were tested in the Morris water maze (MWM). Analysis of maze performance indicated that in sham-injured animals PTZ-produced seizures had a detrimental effect on performance. In injured animals, however, PTZ-treated animals exhibited significantly faster acquisition and better terminal performance in the MWM than did untreated injured animals. These results show that posttraumatic kindled seizures do not exacerbate behavioral deficits after TBI and may, in fact, improve recovery following injury. The findings of this experiment are consistent with the hypothesis that post-TBI neuronal depression may contribute to behavioral morbidity following injury.
本实验的目的是研究实验性创伤性脑损伤（TBI）后损伤后癫痫发作对认知表现的影响。大鼠要么接受中度（2.1个大气压）的中心流体冲击性TBI损伤（n = 16），要么进行手术准备但未接受流体脉冲（假损伤对照，n = 16）。在TBI后24小时开始，受伤动物每天（损伤后第1 - 24天）腹腔注射生理盐水（n = 8）或25 mg/kg戊四氮（PTZ）（n = 8）。假损伤大鼠注射等体积的生理盐水（n = 8）或PTZ（n = 8）。在受伤和假损伤的动物中，每天注射PTZ都会导致行为性癫痫发作的严重程度在数天内增加。在损伤或假损伤后的第25 - 29天，所有动物都在莫里斯水迷宫（MWM）中进行测试。迷宫表现分析表明，在假损伤动物中，PTZ引发的癫痫发作对表现有不利影响。然而，在受伤动物中，接受PTZ治疗的动物在MWM中的习得速度明显更快，最终表现也比未治疗的受伤动物更好。这些结果表明，创伤后点燃性癫痫发作不会加重TBI后的行为缺陷，实际上可能会改善损伤后的恢复。本实验的结果与TBI后神经元抑制可能导致损伤后行为发病的假设一致。
