在植入前使用绿色荧光蛋白（GFP）对转基因胚胎进行快速且非侵入性的筛选。

A rapid and non-invasive selection of transgenic embryos before implantation using green fluorescent protein (GFP).

作者信息

Ikawa M, Kominami K, Yoshimura Y, Tanaka K, Nishimune Y, Okabe M

机构信息

Research Institute for Microbial Diseases, Osaka University, Japan.

出版信息

FEBS Lett. 1995 Nov 13;375(1-2):125-8. doi: 10.1016/0014-5793(95)01162-8.

DOI:10.1016/0014-5793(95)01162-8

PMID:7498460

Abstract

Non-invasive selection of transgenic mice was performed at the stage of preimplantation embryos. The morulae collected from wild female mated with hemizygous transgenic male expressing Aequorea victoria green fluorescent protein (GFP) under chicken beta-actin promoter could be classified as green or non-green under a fluorescent microscope. All the green embryos were shown to carry the transgene by PCR analysis. Taking advantage of the detection of GFP expression can be done non-invasively, the selected embryos were demonstrated to be able to developed to term with 100% of accuracy of the selection.

摘要

在植入前胚胎阶段对转基因小鼠进行非侵入性筛选。从与在鸡β-肌动蛋白启动子控制下表达维多利亚多管水母绿色荧光蛋白（GFP）的半合子转基因雄性交配的野生雌性小鼠收集的桑椹胚，在荧光显微镜下可分为绿色或非绿色。通过PCR分析表明，所有绿色胚胎均携带转基因。利用可非侵入性检测GFP表达这一特性，所选胚胎被证明能够发育至足月，筛选准确率达100%。