大鼠门静脉缩窄后胃黏膜血流灌注的时程变化

Casadevall M, Tabasco-Minguillan J, Panés J, Cirera I, Piqué J M, Terés J

Gastroenterology Department, University of Barcelona, Spain.

J Hepatol. 1995 Aug;23(2):193-8. doi: 10.1016/0168-8278(95)80334-3.

BACKGROUND/AIMS: Gastric mucosal blood flow is increased in all experimental models of chronic portal hypertension, when portosystemic shunting and the hyperdynamic circulation are fully developed. However, some controversy exists concerning the time course of this event. This study was undertaken to investigate the chronological changes in gastric mucosal blood perfusion during the first 7 days after partial portal vein constriction.

METHODS

Portal hypertensive and sham operated animals were studied. Gastric mucosal blood flow was measured by hydrogen-gas clearance and gastric oxygen and hemoglobin content by reflectance spectrophotometry, prior to and immediately after partial portal vein constriction, and 1, 2, 7 and 15 days after induction of portal hypertension.

RESULTS

Immediately after partial portal vein constriction, gastric mucosal blood flow and gastric oxygen were significantly decreased by 27% and 32% respectively, and gastric hemoglobin significantly increased by 19%. On day 1, gastric mucosal blood flow was increased in both portal hypertensive and sham operated rats. However, while this parameter returned to normal in sham operated rats, it remained high on days 2, 7 and 15 in portal hypertensive rats. In portal hypertensive rats, gastric oxygen values were not significantly different from those in sham operated animals, but hemoglobin tended to increase with development of portal hypertension, being significantly higher than in sham operated rats at days 2 and 7. Similar results were obtained when analyzing gastric mucosal blood perfusion 2 and 7 days after two-staged total portal vein constriction.

CONCLUSIONS

Increased gastric mucosal blood perfusion is present in portal hypertensive rats from the first day after partial portal vein constriction. The lack of significant differences in all parameters on day 1 between portal hypertensive and sham operated rats is probably related to an increased mucosal blood perfusion related to surgery.

背景/目的：在所有慢性门静脉高压的实验模型中，当门体分流和高动力循环充分发展时，胃黏膜血流会增加。然而，关于这一事件的时间进程存在一些争议。本研究旨在探讨部分门静脉缩窄后第1至7天胃黏膜血液灌注的时间变化。

方法

对门静脉高压和假手术动物进行研究。在部分门静脉缩窄前、缩窄后即刻以及诱导门静脉高压后1、2、7和15天，通过氢气清除法测量胃黏膜血流，通过反射分光光度法测量胃氧和血红蛋白含量。

结果

部分门静脉缩窄后即刻，胃黏膜血流和胃氧分别显著降低27%和32%，胃血红蛋白显著增加19%。在第1天，门静脉高压和假手术大鼠的胃黏膜血流均增加。然而，虽然该参数在假手术大鼠中恢复正常，但在门静脉高压大鼠中，在第2、7和15天仍保持较高水平。在门静脉高压大鼠中，胃氧值与假手术动物无显著差异，但血红蛋白倾向于随着门静脉高压的发展而增加，在第2天和第7天显著高于假手术大鼠。在分析两阶段完全门静脉缩窄后2天和7天的胃黏膜血液灌注时也获得了类似结果。