Kumaran A K
Differentiation. 1978;12(2):121-5. doi: 10.1111/j.1432-0436.1979.tb00997.x.
Epidermal cells from ligated day-3 last instar larvae of Galleria mellonella secrete a larval cuticle if exposed to ecdysone immediately following ligation; whereas, they deposit a pupal cuticle if exposed to ecdysone three to four days after ligation. During the period of reprogramming the genome in the diploid cells of the wax moth larvae no DNA synthesis occurs. This is direct proof that DNA synthesis is not required for reprogramming in the epidermis of Galleria during larval-pupal transformation.
将大蜡螟3日龄末龄结扎幼虫的表皮细胞在结扎后立即暴露于蜕皮激素时,会分泌幼虫表皮;而在结扎后三到四天暴露于蜕皮激素时,它们会沉积蛹表皮。在蜡螟幼虫二倍体细胞的基因组重编程期间,不发生DNA合成。这直接证明了在大蜡螟幼虫-蛹转化过程中,表皮重编程不需要DNA合成。