大鼠实验性自身免疫性脑脊髓炎的诱导及对脂质结合形式髓鞘碱性蛋白的免疫反应。

Induction of experimental autoimmune encephalomyelitis in rats and immune response to myelin basic protein in lipid-bound form.

作者信息

Massacesi L, Vergelli M, Zehetbauer B, Liuzzi G M, Olivotto J, Ballerini C, Uccelli A, Mancardi L, Riccio P, Amaducci L

机构信息

Department of Neurology, University of Florence, Firenze, Italy.

出版信息

J Neurol Sci. 1993 Oct;119(1):91-8. doi: 10.1016/0022-510x(93)90196-6.

DOI:10.1016/0022-510x(93)90196-6

PMID:7504090

Abstract

Encephalitogenic activity of myelin basic protein (MBP) isolated in a form retaining binding to all myelin lipids was tested in Lewis rats. Immunization with this new stable lipid-bound and native-like preparation (LB-MBP), induced experimental autoimmune encephalomyelitis (EAE) as intensively as the classical lipid free MBP (LF-MBP). During the course of the disease, high affinity specific response to LB-MBP and high frequency of LB-MBP specific precursors was observed in peripheral lymphoid organs, indicating that the disease occurred in presence of anti LB-MBP specific T-cell responsivity. Short term lines, generated from lymphocytes collected at the onset of the disease from LB-MBP immunized rats, showed a strong dose-dependent response to LB-MBP, but not to LF-MBP. The present data indicate that in rat, LB-MBP maintains encephalitogenic activity and induces expansion of a specific T-cell population. These data suggest also that LB-MBP is a new autoantigen that may be relevant in human diseases.

摘要

在Lewis大鼠中测试了以保留与所有髓磷脂脂质结合形式分离的髓磷脂碱性蛋白（MBP）的致脑炎性活性。用这种新的稳定的脂质结合且类似天然状态的制剂（LB-MBP）进行免疫，诱导实验性自身免疫性脑脊髓炎（EAE）的强度与经典的无脂质MBP（LF-MBP）相同。在疾病过程中，在外周淋巴器官中观察到对LB-MBP的高亲和力特异性反应以及LB-MBP特异性前体的高频率，表明该疾病是在存在抗LB-MBP特异性T细胞反应性的情况下发生的。从疾病发作时从LB-MBP免疫大鼠收集的淋巴细胞产生的短期细胞系，对LB-MBP表现出强烈的剂量依赖性反应，但对LF-MBP没有反应。目前的数据表明，在大鼠中，LB-MBP保持致脑炎性活性并诱导特定T细胞群体的扩增。这些数据还表明，LB-MBP是一种可能与人类疾病相关的新自身抗原。