Yamaguchi T, Saneyoshi M, Shudo K
Department of Biological Sciences, Nishi-Tokyo University, Yamanashi, Japan.
Nucleic Acids Symp Ser. 1993(29):135-6.
Inhibitory effects of 2'-deoxy-L-thymidine 5'-triphosphate (L-dTTP) which is an enantiomer of the natural substrate (D-dTTP) on the activity of mammalian DNA polymerases, Escherichia coli DNA polymerase I and HIV-1 reverse transcriptase were examined. Interestingly, L-dTTP showed remarkable inhibitory effect on HIV-1 reverse transcriptase in competitive fashion with respect to the substrate dTTP. In contrast, eukaryotic cell nuclear DNA polymerases alpha and beta were not or slightly inhibited by L-dTTP.
天然底物（D-dTTP）的对映体2'-脱氧-L-胸苷5'-三磷酸（L-dTTP）对哺乳动物DNA聚合酶、大肠杆菌DNA聚合酶I和HIV-1逆转录酶活性的抑制作用进行了研究。有趣的是，L-dTTP以与底物dTTP竞争的方式对HIV-1逆转录酶表现出显著的抑制作用。相比之下，真核细胞核DNA聚合酶α和β未被L-dTTP抑制或仅受到轻微抑制。
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