Imai T, Adachi M, Idaira K, Enomoto T, Matsuura T, Kimura T, Gonogami Y, Mita S, Takahashi T, Tanaka Y
First Department of Internal Medicine, School of Medicine, Showa University.
Arerugi. 1993 Oct;42(10):1585-90.
We studied the effect of indomethacin (IND) on the release of chemical mediators from human peripheral leukocytes and the relation of prostaglandin (PG) E2 and PGI2 to that effect. We stimulated the leukocyte suspensions with calcium ionophore A23187 (CaI, 10(-6) M) accompanied with IND (10(-5) M), PGE2 (10(-5) M) or PGI2 (10(-5) M) and measured the levels in histamine (HA), leukotriene C4/D4/E4 (peptide-LTs) and LTB4 in the supernatant fluid. The increase of HA levels induced by CaI was significantly enhanced by IND (p < 0.01), and the enhancement was significantly inhibited by PGE2 (p < 0.05) and PGI2 (p < 0.01). The release of peptide-LTs was not enhanced by IND and was inhibited significantly by PGE2 (p < 0.05) and PGI2 (p < 0.01). IND did not affect the release of LTB4 induced by CaI. However, LTB4 release was also significantly inhibited by PGE2 and PGI2 (p < 0.01). These results suggest that the inhibitory effect of IND on the production of PGE2 and PGI2 results in the enhancement of HA release. PGE2 and PGI2 have a potent inhibitory effect on the release of HA, peptide-LTs and LTB4 from human leukocytes.
我们研究了吲哚美辛（IND）对人外周血白细胞化学介质释放的影响，以及前列腺素（PG）E2和PGI2与该效应的关系。我们用钙离子载体A23187（CaI，10⁻⁶ M）刺激白细胞悬液，同时加入IND（10⁻⁵ M）、PGE2（10⁻⁵ M）或PGI2（10⁻⁵ M），并测量上清液中组胺（HA）、白三烯C4/D4/E4（肽类白三烯）和LTB4的水平。CaI诱导的HA水平升高被IND显著增强（p < 0.01），而这种增强被PGE2（p < 0.05）和PGI2（p < 0.01）显著抑制。肽类白三烯的释放未被IND增强，且被PGE2（p < 0.05）和PGI2（p < 0.01）显著抑制。IND不影响CaI诱导的LTB4释放。然而，LTB4释放也被PGE2和PGI2显著抑制（p < 0.01）。这些结果表明，IND对PGE2和PGI2产生的抑制作用导致HA释放增强。PGE2和PGI2对人白细胞释放HA、肽类白三烯和LTB4具有强大的抑制作用。
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