Buonomo F C, Klindt J
Monsanto Agricultural Company, Animal Sciences Division, St. Louis, MO 63198.
Domest Anim Endocrinol. 1993 Jul;10(3):257-65. doi: 10.1016/0739-7240(93)90030-f.
Serum GH, IGF-I, IGF-II and IGFBP-2 concentrations were determined by radioimmunoassay in swine of genetic lines which were selected for high (obese) and low (lean) backfat. Blood samples were collected at birth, before and after nursing, at 1 and 3 days of age and at weekly or fortnightly intervals until 30 weeks of age. Overall, GH, IGF-I, IGF-II and IGFBP-2 were highest at birth and declined during the first week of postnatal life. An age-by-line interaction was apparent for GH and IGF-I during the early neonatal period with levels being higher in the lean line than the obese line at 1 day of age and similar at 1 week of age. At 3 to 5 weeks of age there was an elevation in GH which was greater in lean than obese pigs. IGFBP-2 concentration patterns were characterized by a nadir at 5 to 7 weeks of age and a decline from an apex at 8 weeks of age in both lines. IGF-II declined steadily from birth until about 10 weeks of age. A subsequent increase in IGF-II was then observed between 12 and 22 weeks, which was greater in the obese line and in male pigs but not apparent in lean females. At birth, pigs which had not nursed had higher GH and IGFBP-2 and lower IGF-I and IGF-II concentrations. The effect of nursing on IGF-I was significantly influenced by line.(ABSTRACT TRUNCATED AT 250 WORDS)
采用放射免疫分析法测定了选择用于高（肥胖）背膘和低（瘦肉）背膘的遗传系猪的血清生长激素（GH）、胰岛素样生长因子-I（IGF-I）、胰岛素样生长因子-II（IGF-II）和胰岛素样生长因子结合蛋白-2（IGFBP-2）浓度。在出生时、哺乳前后、1日龄和3日龄以及每周或每两周一次直至30周龄时采集血样。总体而言，GH、IGF-I、IGF-II和IGFBP-2在出生时最高，在出生后第一周下降。在新生儿早期，GH和IGF-I存在年龄与品系的相互作用，1日龄时瘦肉型品系的水平高于肥胖型品系，1周龄时相似。在3至5周龄时，GH升高，瘦肉型猪升高幅度大于肥胖型猪。IGFBP-2浓度模式的特点是在5至7周龄时有一个最低点，两个品系在8周龄时从最高点下降。IGF-II从出生到约10周龄稳步下降。随后在12至22周龄之间观察到IGF-II升高，肥胖型品系和雄性猪升高幅度更大，但瘦肉型雌性猪不明显。出生时，未哺乳的猪GH和IGFBP-2较高，IGF-I和IGF-II浓度较低。哺乳对IGF-I的影响受品系的显著影响。（摘要截短至250字）
