一种与CHIP28和WCH-CD水通道同源的新型大鼠肾脏cDNA的克隆。
Cloning of a novel rat kidney cDNA homologous to CHIP28 and WCH-CD water channels.
作者信息
Ma T, Frigeri A, Skach W, Verkman A S
机构信息
Department of Medicine, University of California, San Francisco 94143-0532.
出版信息
Biochem Biophys Res Commun. 1993 Dec 15;197(2):654-9. doi: 10.1006/bbrc.1993.2529.
Two kidney water channels have been identified: CHIP28 in proximal tubule and thin descending limb, and WCH-CD in collecting duct apical membrane. An homologous cDNA (WCH3) was obtained from rat kidney and found to encode a 276 amino acid, 29 kDa protein with 39% amino acid identity to rat CHIP28, 50% to WCH-CD and 49% to MIP26. The WCH3 transcript of 2.5 kb was expressed exclusively in kidney and was upregulated in dehydrated rats. Cell-free translation produced an approximately 28 kDa protein. Analysis of the predicted amino acid sequence indicated a hydrophobic protein with 4-6 membrane-spanning domains, with one N-linked glycosylation site, two conserved NPA boxes common to MIP26 family proteins, and conserved residue C189 common to water channels. WCH3 is a new member of the MIP26 family of channel-forming proteins in mammalian kidney.
已鉴定出两种肾水通道
近端小管和细降支中的CHIP28，以及集合管顶端膜中的WCH-CD。从大鼠肾脏中获得了一种同源cDNA（WCH3），发现它编码一种276个氨基酸、29 kDa的蛋白质，与大鼠CHIP28的氨基酸同源性为39%，与WCH-CD的同源性为50%，与MIP26的同源性为49%。2.5 kb的WCH3转录本仅在肾脏中表达，且在脱水大鼠中上调。无细胞翻译产生了一种约28 kDa的蛋白质。对预测的氨基酸序列分析表明，它是一种具有4 - 6个跨膜结构域的疏水蛋白，有一个N-连接糖基化位点，两个MIP26家族蛋白共有的保守NPA框，以及水通道共有的保守残基C189。WCH3是哺乳动物肾脏中形成通道蛋白的MIP26家族的新成员。
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