碱性和酸性成纤维细胞生长因子的细胞分布、亚细胞定位及可能功能

Cellular distribution, subcellular localization and possible functions of basic and acidic fibroblast growth factors.

作者信息

Eckenstein F P, Kuzis K, Nishi R, Woodward W R, Meshul C, Sherman L, Ciment G

机构信息

Oregon Health Sciences University, Portland 97201.

出版信息

Biochem Pharmacol. 1994 Jan 13;47(1):103-10. doi: 10.1016/0006-2952(94)90442-1.

DOI:10.1016/0006-2952(94)90442-1

PMID:7508717

Abstract

The distribution in the rat nervous system of acidic and basic fibroblast growth factors (FGFs) was analysed by a combination of biochemical and anatomical methods. Acidic FGF (aFGF) was found to be present exclusively in specific neuronal populations, such as motor neurons and basal forebrain cholinergic neurons. Basic FGF (bFGF) was found in astrocytes and in neurons in hippocampal area CA2. Within labelled astrocytes and CA2-neurons, bFGF was detected in both the cytoplasm and the nucleus. The levels of intracellular bFGF were manipulated by antisense oligonucleotide treatment of cultures of developing neural crest cells. Results indicated that the amount of melanogenesis in the cultures is likely to be regulated by intracellular, possibly nuclear bFGF.

摘要

采用生化和解剖学相结合的方法，分析了酸性和碱性成纤维细胞生长因子（FGFs）在大鼠神经系统中的分布情况。发现酸性FGF（aFGF）仅存在于特定的神经元群体中，如运动神经元和基底前脑胆碱能神经元。在星形胶质细胞和海马CA2区的神经元中发现了碱性FGF（bFGF）。在标记的星形胶质细胞和CA2神经元内，在细胞质和细胞核中均检测到bFGF。通过对发育中的神经嵴细胞培养物进行反义寡核苷酸处理，来调控细胞内bFGF的水平。结果表明，培养物中黑色素生成的量可能受细胞内（可能是细胞核内）bFGF的调节。