Tucker R P, Moiseiwitsch J R, Lauder J M
Department of Neurobiology and Anatomy, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157.
Arch Oral Biol. 1993 Dec;38(12):1025-9. doi: 10.1016/0003-9969(93)90163-g.
Tenascin is a large extracellular-matrix glycoprotein found in developing connective tissue. A cDNA probe to mouse tenascin, mTN2, was used to determine the cellular origins of this molecule in the murine tooth germ by in situ hybridization. At embryonic day 19, a hybridization signal significantly greater than background was detected with mTN2 in the subodontoblastic layer of the dental mesenchyme and in the inner enamel epithelium of the enamel organ. At postnatal day 1, a signal was detected over pre-odontoblasts and the strata intermedium and externum. No tenascin mRNA was detected in odontoblasts or the stellate reticulum at either age, and hybridization in ameloblasts was not significantly greater than background at postnatal day 1. Thus, much of the tenascin found throughout developing teeth appears to be synthesized by pre-odontoblasts and the inner enamel epithelium, the two populations of cells destined to generate mineralized matrix.
腱生蛋白是一种在发育中的结缔组织中发现的大型细胞外基质糖蛋白。通过原位杂交，使用针对小鼠腱生蛋白（mTN2）的cDNA探针来确定该分子在鼠牙胚中的细胞起源。在胚胎第19天，在牙间充质的成牙本质细胞下层和牙釉质器官的内釉上皮中，用mTN2检测到明显高于背景的杂交信号。在出生后第1天，在前成牙本质细胞以及中间层和外层检测到信号。在这两个年龄段的成牙本质细胞或星网状层中均未检测到腱生蛋白mRNA，并且在出生后第1天，成釉细胞中的杂交信号并不明显高于背景。因此，在整个发育中的牙齿中发现的大部分腱生蛋白似乎是由前成牙本质细胞和内釉上皮合成的，这两类细胞注定要产生矿化基质。
