Agarwal R, Jugert F K, Khan S G, Bickers D R, Merk H F, Mukhtar H
Department of Dermatology, University Hospitals of Cleveland, Case Western Reserve University, Ohio 44106.
Biochem Biophys Res Commun. 1994 Mar 30;199(3):1400-6. doi: 10.1006/bbrc.1994.1386.
Pyridine, an amphipathic solvent, is widely used in industry and is also a constituent of tobacco and its smoke. Since, in addition to inhalation and ingestion, pyridine is also readily absorbed through skin, we assessed the effect of skin application of pyridine on monooxygenase activities and cytochrome P450 (CYP) isozymes and CYP mRNA levels in the skin of SENCAR mice. Compared to controls, a single topical application of pyridine (30 or 50 mg/100 g body weight) resulted in induction of cutaneous 7-ethoxyresorufin O-deethylase, 7-pentoxyresorufin O-depentylase, and erythromycin N-demethylase activities. Pyridine treatment also resulted in an increase in reactivity with monoclonal antibodies directed against CYP 1A1, 2B1 and 3A. In Northern blot analysis, treatment of pyridine also showed a significant increase in mRNA for Cyp1a-1 in the skin. These data indicate that murine skin contains multiple inducible CYP isozymes, and that pyridine results in the induction of at least three families of CYP in murine skin.
吡啶是一种两性溶剂，在工业中广泛使用，也是烟草及其烟雾的成分之一。由于除了吸入和摄入外，吡啶还易于通过皮肤吸收，我们评估了将吡啶涂抹于皮肤对SENCAR小鼠皮肤中单一加氧酶活性、细胞色素P450（CYP）同工酶及CYP mRNA水平的影响。与对照组相比，单次局部涂抹吡啶（30或50毫克/100克体重）可诱导皮肤7-乙氧基异吩恶唑酮O-脱乙基酶、7-戊氧基异吩恶唑酮O-脱戊基酶及红霉素N-脱甲基酶的活性。吡啶处理还导致与针对CYP 1A1、2B1和3A的单克隆抗体的反应性增加。在Northern印迹分析中，吡啶处理也显示皮肤中Cyp1a - 1的mRNA显著增加。这些数据表明，小鼠皮肤含有多种可诱导的CYP同工酶，且吡啶可导致小鼠皮肤中至少三个CYP家族的诱导。
