Zeilmaker W M, van 't Klooster G A, Gremmels-Gehrmann J F, van Miert A S, Horbach G J
Department of Veterinary Basic Sciences, Utrecht University, The Netherlands.
Biochem Biophys Res Commun. 1994 Apr 15;200(1):120-5. doi: 10.1006/bbrc.1994.1423.
From a liver cDNA library derived from a phenobarbital treated dwarf goat a 1176 bp cDNA-fragment, coding for 285 amino acids, has been isolated. Northern blot analysis reveals detection of a 2 kb mRNA which is inducible by phenobarbital, triacetyloleandomycin and to an lesser extent by beta-naphthoflavone. Analysis of the deduced amino acid sequence shows a 72.5% and a 71.1% homology with rabbit CYP2C3 and human CYP2C17, respectively. The nucleotide sequence reveals homologies of 79.8% and 78.7%, respectively. Comparison of the amino acid sequences between CYP2C forms of various species reveals a high homology around the Cys436, the so-called C-terminal cysteine containing peptide, which is assigned to be the heme-binding region.
从经苯巴比妥处理的矮山羊肝脏cDNA文库中分离出一个1176 bp的cDNA片段，其编码285个氨基酸。Northern印迹分析显示检测到一个2 kb的mRNA，该mRNA可被苯巴比妥、三乙酰竹桃霉素诱导，在较小程度上也可被β-萘黄酮诱导。对推导的氨基酸序列分析表明，它与兔CYP2C3和人CYP2C17的同源性分别为72.5%和71.1%。核苷酸序列的同源性分别为79.8%和78.7%。对不同物种CYP2C形式的氨基酸序列比较显示，在Cys436周围具有高度同源性，Cys436即所谓的含C末端半胱氨酸肽，被认为是血红素结合区域。
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