cDNA and deduced amino acid sequence of a dwarf goat liver cytochrome P450 fragment belonging to the CYP2C gene subfamily.

From a liver cDNA library derived from a phenobarbital treated dwarf goat a 1176 bp cDNA-fragment, coding for 285 amino acids, has been isolated. Northern blot analysis reveals detection of a 2 kb mRNA which is inducible by phenobarbital, triacetyloleandomycin and to an lesser extent by beta-naphthoflavone. Analysis of the deduced amino acid sequence shows a 72.5% and a 71.1% homology with rabbit CYP2C3 and human CYP2C17, respectively. The nucleotide sequence reveals homologies of 79.8% and 78.7%, respectively. Comparison of the amino acid sequences between CYP2C forms of various species reveals a high homology around the Cys436, the so-called C-terminal cysteine containing peptide, which is assigned to be the heme-binding region.