Gerhardt G C, Salisbury C D, MacNeil J D
Agriculture Canada, Health of Animals Laboratory, Saskatoon, SK.
J AOAC Int. 1994 Mar-Apr;77(2):334-7.
A method for the determination of streptomycin and dihydrostreptomycin in pork and bovine muscle and kidney was developed. Dilute perchloric acid solution is used to precipitate proteins and extract the analytes from the tissue. The extract is loaded onto a cation-exchange, solid-phase extraction column, and the drugs are eluted with pH 8 phosphate buffer. The eluant is chromatographed by using an on-line column enrichment liquid chromatographic system with postcolumn derivatization using 1,2-naphthoquinone-4-sulfonic acid and detection by fluorescence. The recoveries were 61.1% (coefficient of variation [CV], 7.3%) for streptomycin and 55.3% (CV, 8.2%) for dihydrostreptomycin. The detection limits were 10 ppb for streptomycin and 20 ppb for dihydrostreptomycin.
开发了一种测定猪肉、牛肉肌肉和肾脏中链霉素和双氢链霉素的方法。用稀高氯酸溶液沉淀蛋白质并从组织中提取分析物。将提取物加载到阳离子交换固相萃取柱上，并用pH 8的磷酸盐缓冲液洗脱药物。使用在线柱富集液相色谱系统对洗脱液进行色谱分析，柱后用1,2-萘醌-4-磺酸衍生化，荧光检测。链霉素的回收率为61.1%（变异系数[CV]，7.3%），双氢链霉素的回收率为55.3%（CV，8.2%）。链霉素的检测限为10 ppb，双氢链霉素的检测限为20 ppb。
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