用单光子发射计算机断层扫描（SPECT）对钴-57-博来霉素进行定量分析，以预测肺癌患者的治疗反应和预后。

SPECT quantitation of cobalt-57-bleomycin to predict treatment response and outcome of patients with lung cancer.

作者信息

Even-Sapir E, Bettman L, Iosilevsky G, Milshtein D, Frenkel A, Kolodny G M, Ben-Haim S, Israel O, Front D

机构信息

Department of Nuclear Medicine, Rambam Medical Center, Haifa, Israel.

出版信息

J Nucl Med. 1994 Jul;35(7):1129-33.

PMID:7516965

Abstract

UNLABELLED

Our hypothesis is that the concentration of 57Co-bleomycin (Co-bleo) in lung tumors reflects tumor cell kinetics and thus, prognosis. The relationship between the tumor concentration of Co-bleo measured in vivo by quantitative SPECT, response to chemotherapy and survival was investigated.

METHODS

Twenty patients with small-cell lung carcinoma (SCLC) and 49 patients with non-small-cell lung carcinoma (NSCLC) were studied. The concentration of Co-bleo was measured by SPECT in vivo in the tumor. The correlation between Co-bleo concentration in the tumor and the fraction of Co-bleo bound to DNA was investigated in an EMT6 murine tumor model and in samples of eight human tumors.

RESULTS

Tumors that did not respond to treatment showed a significantly higher Co-bleo concentration 8 hr after injection than tumors that responded (5.83% +/- 1.97% ID/cc * 10(-3) versus 2.55% +/- 1.23% ID/cc * 10(-3), p < 0.001). Values of Co-bleo concentration of 2.97% ID/cc * 10(-3) for SCLC and 2.72% ID/cc * 10(-3) for NSCLC were found to best separate patients into short- and long-term survival groups. In the EMT6 murine tumor model, a good correlation was found between the concentration of Co-bleo in the tumor and the fraction of Co-bleo bound to DNA (r = 0.75). In human tumor samples, a good correlation was found between DNA-bound Co-bleo measured in vitro and the concentration measured in vivo by SPECT (r = 0.85).

CONCLUSIONS

SPECT-measured Co-bleo concentration predicts the response to treatment and the outcome in patients with lung tumor by showing Co-bleo binding to DNA and tumor cell kinetics.

摘要

未标记

我们的假设是，肺肿瘤中57钴-博来霉素（Co-博来霉素）的浓度反映肿瘤细胞动力学，进而反映预后。研究了通过定量单光子发射计算机断层扫描（SPECT）在体内测量的Co-博来霉素肿瘤浓度、化疗反应与生存之间的关系。

方法

对20例小细胞肺癌（SCLC）患者和49例非小细胞肺癌（NSCLC）患者进行了研究。通过SPECT在体内测量肿瘤中Co-博来霉素的浓度。在EMT6小鼠肿瘤模型和8例人类肿瘤样本中研究了肿瘤中Co-博来霉素浓度与结合到DNA上的Co-博来霉素比例之间的相关性。

结果

对治疗无反应的肿瘤在注射后8小时的Co-博来霉素浓度显著高于有反应的肿瘤（5.83%±1.97% ID/cc×10⁻³ 对 2.55%±1.23% ID/cc×10⁻³，p<0.001）。发现SCLC的Co-博来霉素浓度值为2.97% ID/cc×10⁻³，NSCLC为2.72% ID/cc×10⁻³时，最能将患者分为短期和长期生存组。在EMT6小鼠肿瘤模型中，肿瘤中Co-博来霉素的浓度与结合到DNA上的Co-博来霉素比例之间发现良好的相关性（r = 0.75）。在人类肿瘤样本中，体外测量的结合到DNA上的Co-博来霉素与SPECT在体内测量的浓度之间发现良好的相关性（r = 0.85）。