Broll R, Kayser K, Vollmer G, Plagemann M, Bruch H P
Klinik für Chirurgie, Medizinische Universität, Lübeck.
Zentralbl Pathol. 1994 Jul;140(2):123-8.
Tenascin, a glycoprotein of the extracellular matrix, is involved in epithelial proliferation, differentiation and cell migration during embryonic development. Later on, it is important in proliferative processes like tumor growth and wound healing. We examined the distribution of tenascin, especially in the stroma near basement membranes, in paraffin sections from 25 patients with gastric cancer by immunohistological staining with regard to tumor staging, grading and Lauren classification. In two series we used a monoclonal and a polyclonal antibody against human tenascin. In most of the sections there was a more intensive staining of the surrounding stroma in areas close to the tumor, which could indicate the direction of tumor growth. In highly differentiated tumors we found a positive, yet discontinuous staining close to the basement membrane. In contrast to this, we saw a more diffuse reaction of the stroma with network-like staining of the collagen fibres in undifferentiated tumors. In lymph nodes we also detected a small number of tumor cells by staining of the surrounding stroma.
腱生蛋白是一种细胞外基质糖蛋白，在胚胎发育过程中参与上皮细胞的增殖、分化和细胞迁移。后来，它在肿瘤生长和伤口愈合等增殖过程中发挥重要作用。我们通过免疫组织化学染色，对25例胃癌患者石蜡切片中腱生蛋白的分布进行了研究，尤其关注其在基底膜附近基质中的分布情况，并分析了其与肿瘤分期、分级及劳伦分类的关系。在两个系列实验中，我们分别使用了抗人腱生蛋白的单克隆抗体和多克隆抗体。在大多数切片中，靠近肿瘤区域周围的基质染色更为强烈，这可能提示肿瘤生长的方向。在高分化肿瘤中，我们发现靠近基底膜处有阳性但不连续的染色。与此相反，在未分化肿瘤中，我们看到基质有更弥漫的反应，胶原纤维呈网状染色。在淋巴结中，通过对周围基质染色，我们也检测到了少量肿瘤细胞。
